厦门大学海洋与地球学院

College of Ocean and Earth Sciences
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叶海辉教授课题组解读MF调控青蟹卵巢发育的信号通路
海洋与地球学院 海洋与地球学院 2016/3/24 4568 返回上页

生殖是生物体的基本特征之一,也是生命科学领域长期受到关注的研究方向之一。甲壳动物大颚器分泌的甲基法尼酯(MF)为昆虫保幼激素Ⅲ的非环氧化形式,参与了蛋白质代谢、生殖、蜕皮与变态等生理过程,迄今MF参与生殖调控的信号通路尚不清楚。我院叶海辉教授课题组对我国重要海洋蟹类——拟穴青蟹开展了研究,在查明MF可能的受体(维甲酸X受体,SpRXR)基因序列、时空表达、mRNA定位的基础上,采用体外培养系统和RNA干扰等方法,进一步分析了MF的调控途径。研究发现MF对保幼激素信号通路相关基因没有明显诱导作用,但能够显著诱导SpRXR和卵黄蛋白原(SpVg)的表达,表明MF可能通过SpRXR促进卵巢发育。此外,发现SpRXR参与了蜕皮激素信号通路,但是MF并没有共用该通路的相关因子。该研究较为详细地揭示了甲壳动物MF的作用途径,有助于深入理解卵巢发育与生殖的分子机制,同时为养殖技术提供了理论指导。

该研究成果近日发表在在Scientific Reports期刊上。论文第一作者为2012级博士生巩杰,在叶海辉教授和曾朝曙教授共同指导下完成。

Figure 1. The possible mechanism and signaling pathway of MF and RXR on regulating ovarian development.

Solid arrows indicate positive for the expressions of responses genes; question mark indicates uncertain factors; dashed arrow means unproved signaling pathway; Solid arrows with × represent no sharing of the related signaling pathway. YO: Y-organ, MO: mandibular organ, CA: corpora allata.

Citation: Gong J, Huang CC, Shu L, Huang HY, Ye HH*, Zeng CS*, Li SJ. The retinoid X receptor (RXR) from the mud crab: new insight into its role in the ovarian development and related signaling pathway. Scientific Reports, 2016, 6: 23654; DOI: 10.1038/srep23654

Abstract:

In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean reproduction are poorly understood. In the present study, the RXR mRNA was detected in the ovarian follicular cells of mud crab Scylla paramamosain (SpRXR) and during ovarian maturation, its expression level was found to increase significantly. In vitro experiment showed that both SpRXR and vitellogenin (SpVg) mRNA in the ovarian explants were significantly induced by 20-hydroxyecdysone (20E) but not methyl farnesoate (MF). However, differing from the in vitro experiment, injection of MF in in vivo experiment significantly stimulated the expressions of SpRXR and SpVg in female crabs at early vitellogenic stage, but the ecdysone and insect juvenile hormone (JH) signaling pathway genes were not induced. The results together suggest that both MF and SpRXR play significant roles in regulating the expression of SpVg and ovarian development of S. paramamosain through their own specific signaling pathway rather than sharing with the ecdysone or the insect JH.

其他相关阅读:

Bao CC, Yang YN, Huang YH, Ye HH*. Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis. Scientific Reports, 2015, 5:17055, DOI: 10.1038/srep17055